;1.9) Program: CH2 spectral editing by selection of three-spin coherence ;Description: Selects CH2 (methylene) signals ;Requires: !!!! first set-up; !!! run routinely (ca. 6% effic.). Higher power during tr = ;140 us; 5.8 kHz MAS ;Reference(s): Mao & KSR, unpublished ;srcpCH2selmrevA.90T1 ;from srcpCH2selmrevA.90 WORKS WELL (5.6%) ;from srcpCH2selmrev.90 ;from srcpCH2selmrev.tppm ;pl2 is power level for contact, pl12 is power level for decoupling on F2 ;"d13=1s/l31-p2/2-2u" define delay trot "trot = 31*p11+p21+15*d8+2*p23+1u+p2/2" "d8=p11" ;MREV-8 window "d9=d8/2" "d17=7*p11+p21+d9+3*d8-4u-p2/2" ;1 MREV-8 cycle (C-H excitation) "d18=1s/l31-2u-3u-p2/2" ;1 tr "d28=1s/l31-2u-3u-p2/2-7*p11-d9-3*d8" ;1 tr - 1 MREV-8 cycle "d27=1s/l31-2u-3u-p2/2-p1/2" 1 ze trot 2 d1 do:f2 ;recycle delay 10u pl2:f2 ;preselect pl2 drive power for F2 10u pl11:f1 ;preselect pl1 drive power level for F1 p1:f1 ph9^ ;destroy 13C magnetization d20 p1:f1 ph9^ d20 p1:f1 ph9^ d20 p1:f1 ph9^ d20 10u pl1:f1 10u fq1:f2 ;on resonance for CP 1u:f2 ph1 1u:f1 ph2 ;--------------- start real pulse sequence --------------------- p3:f2 ph1 ;proton 90 pulse ; d19:f2 ph10 ;ca. 1u: allow time for the phase shift (p15 ph2):f1 (p15 ph10):f2 ;contact pulse 1u cw:f2 1u pl12:f2 d18 pl11:f1 ;1 tr 3u pl22:f2 ; tr p2:f1 ph5 ;180 C with exorcycle 3u 1u pl12:f2 d28 cpds2:f2 ;1 tr - MREV-8 cycle 1u do:f2 d9 pl22:f2 fq2:f2 ;near resonance for MREV-8 p11:f2 ph22 ;2 MREV-8 p11:f2 ph21 ;y d8 p11:f2 ph23 ;3 p11:f2 ph20 ;0 d8 p11:f2 ph20 ;0 p11:f2 ph21 ;y d8 p11:f2 ph23 ;3 (p11 ph22):f2 (p21 ph6):f1 ;13C 90 to remove Sy Iz terms ;................ 2 tr ..... special pulses ..................... ;composite 1H 0/90 y or -y, made from 45-degree pulses p23:f2 ph11 ;45 pulse p23:f2 ph12 p11:f2 ph22 ;2 p11:f2 ph21 ;y d8 p11:f2 ph23 ;3 p11:f2 ph20 ;0 d8 p11:f2 ph20 ;0 p11:f2 ph21 ;y d8 p11:f2 ph23 ;3 p11:f2 ph22 ;2 ; tr/2 d8 p11:f2 ph22 ;2 p11:f2 ph21 ;y d8 p11:f2 ph23 ;3 p11:f2 ph20 ;0 d8 p11:f2 ph20 ;0 p11:f2 ph21 ;y d8 p11:f2 ph23 ;3 p11:f2 ph22 ;2 d8 p11:f2 ph22 ;2 p11:f2 ph21 ;y d8 p11:f2 ph23 ;3 p11:f2 ph20 ;0 d8 p11:f2 ph20 ;0 p11:f2 ph21 ;y d8 p11:f2 ph23 ;3 p11:f2 ph22 ;2 d9 fq1:f2 1u cw:f2 ;turn on F2 decoupling d17 ;1 MREV-8 cycle 3u pl22:f2 p2:f1 ph3 ;13C 180 for Hahn echo 3u 1u pl12:f2 d27 ;tr p1:f1 ph7 d6 do:f2 ;filter to reduce dispersive components (>10 ms) and CH3 (13C T1) 1u cw:f2 1u pl12:f2 p1:f1 ph8 d13 3u pl22:f2 p2:f1 ph2 ;13C 180 for Hahn echo 3u 1u pl12:f2 ;set decoupling drive power level 1u cpds2:f2 ;turn on F2 decoupling d3:f1 ph0 ;dead time delay, reset RF phase to detec go=2 ph31 1m do:f2 ;turn decoupler off wr #0 exit ph0= 0 ph1= 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ;1H 90 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 ph2= 0 0 0 0 0 0 0 0 ;13C CP 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 ph3= 0 0 0 0 0 0 0 0 ;180 13C before detection 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 0 0 0 0 0 0 0 0 ; 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 1 1 1 1 1 1 1 1 ;exorcycle 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 0 0 0 0 0 0 0 0 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 3 3 3 3 3 3 3 3 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 ph5= 0 1 2 3 0 1 2 3 ;prefocus 180 on C with exorcycle 1 2 3 0 1 2 3 0 ;exorcycle 2 3 0 1 2 3 0 1 3 0 1 2 3 0 1 2 ph6= 0 0 0 0 0 0 0 0 ;90 13C remove SyIz coherence 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 ph7= 1 1 1 1 1 1 1 1 ;store for 13C T1 filter 2 2 2 2 2 2 2 2 ph8= 3 3 3 3 3 3 3 3 ;read out from 13C T1 filter 0 0 0 0 0 0 0 0 ph9= 0 1 ;destroy 13C magnetization ph10= 0 ;1H CP ph11= 1 1 1 1 1 1 1 1 ;1st 45 H (0/90) ph12= 3 3 3 3 1 1 1 1 ;2nd 45 H ph20=0 ;MREV-8 ph21=1 ph22=2 ph23=3 ph31= 0 2 0 2 2 0 2 0 ;RCPH: 2nd two inverted for exorcycle, ;2nd four inverted for Sx subtraction (IzJz dephased) 1 3 1 3 3 1 3 1 ;CP phase moves in 90 steps 2 0 2 0 0 2 0 2 3 1 3 1 1 3 1 3 2 0 2 0 0 2 0 2 ;1H first 90 inverted 3 1 3 1 1 3 1 3 0 2 0 2 2 0 2 0 1 3 1 3 3 1 3 1 2 0 2 0 0 2 0 2 ;exorcycle of detection pulse 3 1 3 1 1 3 1 3 0 2 0 2 2 0 2 0 1 3 1 3 3 1 3 1 0 2 0 2 2 0 2 0 1 3 1 3 3 1 3 1 2 0 2 0 0 2 0 2 3 1 3 1 1 3 1 3